Micro-Rheology of Biological and Bio-Artificial Fluids
DOI:
https://doi.org/10.31265/q5k1fv71Abstract
Microrheology is a quite new, but interesting technique in the biological field. This non-destructive measuring methods infers elastic and viscous flow parameters from the motion of embedded tracer particles using the generalized Stokes-Einstein relation. It is offering access from 1 to 10.000 Hz with small sample volumes (µl) and cellular-scale resolution. This study has been focusing on the viscosity of different biological fluids like cerebrospinal fluid (CSF, human), synovial fluid (SF, dog, horse) and artificial CSF and SF.
The viscoelastic behavior of the studied samples is largely attributable to their protein content. To test this, artificial cerebrospinal fluid (CSF) and synovial fluid (SF) as well as their individual components were prepared and analyzed, using the same parameters as for corresponding biological samples. To validate the method, measurements were compared with macro-rheological data and demonstrated that the results from both approaches are complementary1,2,6,8.
References
Yating, M.; Paige, N.; Jamel, A.; Passive and Active Microrheology for Biomedical Systems. Frontiers in Bioengineering and Biotechnology 2022, 10:916354. https://doi.org/10.3389/fbioe.2022.916354
Bradt, E.; Hild, S.; Reichel, E.K.; Kracalik, M. Microrheology of Biofluids. In Book of Abstracts Annual European Rheology Conference, Sorrento, Italy, 17-18 April 2018; European Society of Rheology: Zurich, Switzerland, 2018.
Tomaiuolo, G.; Carciati, A.; Caserta, S.; Guido, S. Blood linear viscoelasticity by small amplitude oscillatory flow. Rheol. Acta 2016, 55, 485-495. https://doi.org/10.1007/s00397-015-0894-3
Barrand, M.; Hladky, S. Mechanisms of fluid movement into, through and out of the brain: Evaluation of the evidence. Fluids Barriers CNS 2014, 11, 26. https://doi.org/10.1186/2045-8118-11-26
Ropper, A.H.; Samuels, M.A.; Klein, J.P. Adams & Victor's Principles of Neurology, 10th ed.; McGraw-Hill Education: New York, NY, USA, 2014; pp. 615-638.
Bauer, T.; Bradt, E.; Hild, S.; Gruber, A.; Rossmann, T.; Navarro, F.; Oberndorfer, J.; Stefanits, H.; Kracalik, M.; Analysis of interactions in cerebrospinal fluid under different temperature conditions using rotational rheometry, Biorheology 59 (2022/2023) 99-112.
Bauer, T.; Bradt, E.; Hild, S.; Gruber, A.; Rossmann, T.; Navarro, F.; Oberndorfer, J.; Stefanits, H.; Kracalik, M.; Rheological Characterization of Cerebrospinal Fluid Under Different Temperature Conditions, Fluids 2026, 11 (2), 38. https://doi.org/10.3390/fluids11020038
Bradt, E.; Bauer, T.; Hild, S.; Gruber, A.; Rossmann, T.; Navarro, F.; Oberndorfer, J.; Stefanits, H.; Kracalik, M.; Application of macro- and microrheology for biological fluids, Biorheology 59 (2022/2023) 99-112. 9.
Addis, A.; Gaasch, M.; Schiefecker, A.J.; Kofler, M.; Ianosi, B.; Rass, V.; Lindner, A.; Broessner, G.; Beer, R.; Pfausler, B.; et al. Brain temperature regulation in poor-grade subarachnoid hemorrhage patients-A multimodal neuromonitoring study. J. Cereb. Blood Flow Metab. 2021, 41, 359-368. https://doi.org/10.1177/0271678X20910405
Kunitsyn, V.G.; Panin, L.E.; Polyakov, L.M. Anomalous change of viscosity and conductivity in blood plasma lipoproteins in the physiological temperature range. Int. J. Quantum Chem. 2001, 81, 348-369. https://doi.org/10.1002/1097-461X(2001)81:5<348::AID-QUA1004>3.3.CO;2-8
Michieletto, D.; Marenda, M. Rheology and Viscoelasticity of Proteins and Nucleic Acids Condensates. JACS Au 2022, 2, 1506-1521.
https://doi.org/10.1021/jacsau.2c00055
Weihs, D.; Mason, T. G.; Teifel, M. A.; Bio-Microrheology: A Frontier in Microrheology, Biophysical Journal, Volume 91, 4296-4305. https://doi.org/10.1529/biophysj.106.081109
Bortel, E.L.; Charbonnier, B.; Heuberger, R.;Development of a Synthetic Synovial Fluid for Tribological Testing, Lubricants 3 (4), 2015, S. 664-686 https://doi.org/10.3390/lubricants3040664
Wilhelm F.; Krause, M.; Schob S.;Merkenschlager, A.; Wachowiak, R.;Härtig, W.; Meixenberger, J.; Gburek-Augustat, J.; Wende, T.; Cerebrospinal Fluid Protein Concentrations in Hydrocephalus, Children 2023, 10(4), 644. https://doi.org/10.3390/children10040644
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Elke Bradt, Thessa-Carina Bauer, Sabine Hild, Andreas Gruber, Tobias Rossmann, Francisco Ruiz-Navarro, Johannes Oberndorfer, Harald Stefanits, Thomas Voglhuber-Brunnmaier, Alexander O. Niedermayer, Milan Kracalik
This work is licensed under a Creative Commons Attribution 4.0 International License.